Question: What Does FITC Mean?

What wavelength is DAPI?

358 nmFluorescence properties When bound to double-stranded DNA, DAPI has an absorption maximum at a wavelength of 358 nm (ultraviolet) and its emission maximum is at 461 nm (blue).

Therefore, for fluorescence microscopy, DAPI is excited with ultraviolet light and is detected through a blue/cyan filter..

What color is PE fluorescence?

R-phycoerythrin (R-PE) is an intensely bright phycobiliprotein isolated from red algae that exhibits extremely bright red-orange fluorescence with high quantum yields. It is excited by laser lines from 488 to 561 nm, with absorbance maxima at 496, 546, and 565 nm and a fluorescence emission peak at 578 nm.

Is FITC green?

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. … FITC has excitation and emission spectrum peak wavelengths of approximately 495 nm/519 nm, giving it a green color. Like most fluorochromes, it is prone to photobleaching.

What does FITC bind to?

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins vi a the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.

Is Fluorescein the same as FITC?

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. … FITC has excitation and emission spectrum peak wavelengths of approximately 495 nm/519 nm, giving it a green color. Like most fluorochromes, it is prone to photobleaching.

What is PE flow cytometry?

R-PE is a large molecule used for fluorescence-based detection, primarily in flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity but not photostability.

What is FITC used for?

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins vi a the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.

What is FITC and PE?

The FITC / PE Compensation Standard is to be used in conjunction with hardware or software to remove spectral overlap from fluorochromes into secondary fluorescence detectors of a flow cytometer. … The FITC/PE Compensation Standard is a mixture of 4 populations of microspheres: FITC, PE, FITC/PE, and AutoFluor™.

What Colour is FITC?

The blue color spectrum is 450-495 nm, and the green spectrum is 495-570 nm. Fluorescein isothiocyanate (FITC) is the derivative of fluorescein with an isothiocyanate reactive group, rendering it reactive towards amine and sulfhydryl groups commonly found in biomolecules.

At what wavelength does GFP fluorescence?

510 nmGreen Fluorescent Protein (GFP) Green Fluorescent Protein (GFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. GFP can be excited by the 488 nm laser line and is optimally detected at 510 nm.

What wavelength is FITC?

FITC emits fluorescence from 475 to 650 nm, peaking at 525 nm, which falls in the green spectrum.

Does DAPI stain dead cells?

DAPI (4′,6-diamino-2-phenylindole, dihydrochloride) is a fluorescent nucleic acid stain that binds to minor grove A-T rich regions of double-stranded DNA. It is essentially excluded from viable cells, but can penetrate cell membranes of dead or dying cells.

Can I use PE and PE cy7 together?

When using tandem antibody conjugates in multicolor staining panels, it is important to use exactly the same tandem conjugate for compensation tubes that are used for staining experiment samples. … PE-Cy7 (BD) ≠ PE-Cy7 (Biolegend) for any antibody conjugate. CD4 PE-Cy7 (BD) ≠ CD8 PE-Cy7 (BD) for compensation.

What color is PE?

488 nm 532 nm 561 nm 575/26 PE (Exmax 496 nm/Emmax 578 nm) R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae.

Can I use APC cy7 and PE cy7 together?

Most recent answer. In my experience, it is not advisable to use APC/Cy7 and PE/Cy7 together due to heavy cross-beam contamination as their emission max are exactly same. However, they can be used together if you are using machines like the Cytek Aurora.